HiScale™ column design for medium to high pressure liquid chromatography
HiScale™ pressure‐stable, empty columns are designed for process development and preparative chromatography using ÄKTA protein purification systems. These chromatography columns withstand pressures up to 20 bar, with a user‐friendly design that supports process optimization through simplified and reproducible operations.
The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments as well as disassembly and cleaning. The entire shaft may be rinsed after column packing, minimizing the risk of bacterial growth. The column’s measurement scale helps users to predict and reach a predefined bed height.
HiScale™ columns are based on the well‐established line of XK columns. They are compatible with all methods currently applicable to XK columns.
Viral clearance and other scaledown studies with HiScale™ 10/40
HiScale™ 10/40 columns are especially suitable for scaledown studies used in, for example, viral clearance evaluation in downstream processing. The small inner diameter and flexible adapters minimize costly sample and viral spike volumes. The robust design of HiScale™ 10/40 allows for controlled bed heights and gives reproducible results offering an effective scaled down model of the large-scale downstream process.
Resin compatibility for medium to high pressure liquid chromatography
HiScale™ chromatography columns are compatible with all Cytiva resins. For columns packed with SOURCE 15 and SOURCE 30, a 10 µm net ring should be used.
ÄKTA system and other chromatography system compatibility
The user-friendly design of the HiScale™ column facilitates trouble-free operation, with fittings for direct connection to ÄKTA systems. These empty chromatography columns are also compatible with other high performance liquid chromatography systems that have the appropriate pressure capacity.
| Bed height with two adapters |
0 to 200 mm |
| Bed support |
Net |
| Chemical stability |
The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol. Solutions containing more than 2 M NaOH, 1 M HCl, other strong mineral acids, or 75% acetic acid should not be used. The column may be used at temperatures from 4°C to 40°C and at pressures up to 20 bar (2 MPa, 290 psi). |
| Inlet i.d. |
1 mm (0.04 in) |
| Material (column hardware) |
Borosilicate glass, Polycarbonate, Polyamide (reinforced glass fiber), Ethylene propylene diene polymer (EPDM), Polyoxymetylen (POM), Polyether ether ketone (PEEK), Ethylenetetrafluoroethylene (ETFE), Fluoro-rubber (Viton™), Polypropylene, Polyamide (nylon). |
| Material (bed support) |
Polypropylene (PP) (reinforced glass fiber), Polyamide (PA). |
| Material (column tube) |
Borosilicate glass, Polycarbonate, Polyamide (reinforced glass fiber), Ethylene propylene diene polymer (EPDM), Polyoxymetylen (POM), Fluoro-rubber (Viton™), Polypropylene, Polyamide (nylon). |
| Porosity |
20 μm |
| Pressure limit |
20 bar (2 MPa, 290 psi) |
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